Endocrine Abstracts

Testosterone is a well established, safe, effective and affordable hormone for use in androgen replacement therapy (ART) for androgen deficiency (AD) due to pathology of the hypothalamo-pituitary testicular axis. Newer testosterone products provide short-acting (transdermal) or long-acting (depot injectable) delivery with enhanced convenience to improve long-term compliance during life-long ART. Yet there is a striking disparity between major under-diagnosis of authentic AD (e...

Recent in-vitro studies have shown that vitamin D metabolites undergo conjugation reactions by sulfation and glucuronidation and may be important mechanisms for the inactivation, storage and excretion of analytes. However the circulating concentrations and clinical significance of phase II vitamin D conjugation is unclear as current analysis has almost exclusively been restricted to measuring their unconjugated metabolite forms. In this study we aimed to develop an analytical ...

Of 563 genes involved in multiple processes of development of hypothalamo-pituitary-gonadal (HPG) axis during embryogenesis, mutations in 62 genes cause delayed puberty. We selected 6 of 37 delayed puberty patients for whole exome sequencing (WES) based on advanced age (>18 years) and severity of disease symptoms. Genomic DNA extraction was done using Qiagen kit at Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan. DNA samples were taken to ANZAC Researc...